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KMID : 0381120080300030215
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Genes and Genomics
2008 Volume.30 No. 3 p.215 ~ p.222
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Genetic Variation and Divergence in Two Critically Endangered Mahseer Species Tor tor and Tor putitora (Pisces : Cypriniformes) Assessed by RAPD Markers
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Ghosh Debashish
Alam Md. Samsul
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Abstract
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Tor tor and Tor putitora are two important cyprinid species distributed in many countries of the South and South East Asia. The species are critically endangered in Bangladesh. Therefore information on genetic structure of the species would be useful to take management plan for conservation and stock enhancement of these two species. Random Amplified Polymorphic DNA (RAPD) markers were used to assess the genetic variation in these species. Upon screening of 27 random decamer primers six primers giving consistent banding patterns with good resolution were selected for the analysis of twelve fish of each species. The primers generated 44 loci of which 41 were polymorphic. The frequency of polymorphic loci was 86.36% in T. tor and 31.83% in T. putitora. The band-sharing based genetic similarity of T. tor was lower than that in T. putitora. The gene diversity in T. tor was 0.270 while that in T. putitora was 0.106. The genetic identity
and genetic distance values between the two species were 0.863 and 0.147 respectively. The population differentiation value (PhiPT) between the two species was highly significant (P <0.001). No species diagnostic bands were detected in either of the species though fifteen private bands, 13 for T. tor and two for T. putitora were obtained. The present study detected higher levels of genetic variability in T. tor compared to T. putitora.
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KEYWORD
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genetic variation, mahseer, RAPD, Tor tor, Tor putitora
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